Fermentas ta cloning kit manual

TOPO TA cloning of ompA into pBAD-TOPO vector. The ompA PCR product (4 µL) was cloned into the pBAD vector, as outlined by manufacturer’s instructions, using the pBAD TOPO TA® Expression Kit (Invitrogen, Cat. #K). Screening of pBAD insert by PCR amplification. Whole colony PCR was performed on the selected transformants to specifically. Kit Contents and Storage Shipping and ® storage The TA Cloning Kits are shipped on dry ice and contain a box of TA Cloning ® Reagents (Box 1) and a box of ®One Shot Competent Cells (Box 2). Catalog nos. K and K are not supplied with One Shot ® Competent Cells. Store Box 1 at −30°C to −10°C in a non-frost-free freezer andFile Size: KB. was cloned using a PCR Cloning Kit (pCR®8/GW/ TOPO ® TA Cloning Kit, Invitrogen, Paisley, UK), sequenced, and compared with database sequences using the BLAST program from NCBI. cDNAs were synthesized from RNA samples according to the manual of the RevertAid™, First Strand cDNA Synthesis Kit from Fermentas (Fermentas, Cornaredo, Italy).

The TA Cloning Kit with pCR 2 1 vector provides a quick one step cloning strategy for directly inserting a Taq amplified PCR product into a plasmid vector The TA Cloning Kit uses the pCR 2 1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen minute room temperature ligation step Reactions typically yield 80 recombinants containing inserts Features of the. fermentas ta cloning kit | سفیر آزما کیان مرجع فروش مواد شمیایی و آزمایشگاهی در ایران برای سفارش با تلفن در تماس باشید. InsTAclone™ PCR Cloning Kit. Catalog Number.: K Description.: The InsTAcloneâ„¢ PCR Cloning Kit is a TA system for direct one-step cloning of PCR products with 3'-dA overhangs. Overhangs of this type are generated by Taq DNA polymerase and other thermostable DNA polymerases which lack proofreading activity.

5 thg 6, See page 15 for a protocol. Alternatively, you may want to try the Zero Blunt® PCR Cloning Kit (see page 19 for ordering information). This kit. 21 All components of the kit were function tested in control experiment as described in the manual. A µL aliquot of the ligation mixture. The following protocol is provided for your convenience. Other protocols are suitable. 1. Prepare a PCR cocktail consisting of PCR buffer, dNTPs, primers, and.